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. 2017 Nov 1;7:14791. doi: 10.1038/s41598-017-14818-7

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Physico-chemical characterization of AtuPLEX2. AtuPLEX2 lipoplex is composed of 70 mol% cationic lipid AtuFECT01, 1 mol% CerC8 PEG, 29% cholesterol and blunt ended siRNA duplexes with alternating modifications on both strands. (A) Size distribution:Z-average size of 29 nm as determined by dynamic light scattering (intensity distribution), measured in 270 mmol/L sucrose buffer, in triplicates. (B) The Zeta potential of representative lipoplexes has iis peak at 42.1 mV, measured in 270 mmol/L sucrose buffer, in triplicates.