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. 2017 Nov 1;7:14798. doi: 10.1038/s41598-017-14941-5

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Change in speckle contrast occurs seconds after blood supply ligation. A noticeable change in speckle contrast (b,d) was observed in the same parathyroid gland seconds after tying off blood supply to the gland, however no such change was observed in white light images (a,c). Images (a) and (b) were taken before, while images (c) and (d) were taken about 30 seconds after ligation of the blood supply. Parathyroid gland is indicated with a white ellipse.