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. 2017 Nov 1;7:14882. doi: 10.1038/s41598-017-14922-8

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Statistical significance of R-G IF with different data sizes (a) A heat map showing the mean Student’s t-test p-value that resulted from the comparison of the calculated R-G IFs of simulated experimental groups with 5 images/group for a range of R-G IFs (values indicate the mean p-value for 20 comparisons). (b) A heat map showing the mean Student’s t-test p-value that resulted from the comparison of the calculated R-G IFs of simulated experimental groups with 10 images/group for a range of IFs (values indicate the mean p-value for 20 comparisons). (c) A heat map showing the mean Student’s t-test p-value that resulted from the comparison of the calculated R-G IFs of simulated experimental groups with 20 images/group for a range of R-G IFs (values indicate the mean p-value for 20 comparisons).