Fig. 4.

MSI overexpression increases migration and invasion of MCF7 and MDA-MB-231 MCF7-expressing GFP and MSI2-GFP were counted by Vi-cell-XR, and equally plated on transwells with and without Matrigel. Migration a and invasion b qualities were assessed by counting cells 48 h after initial plating. MSI2 overexpression causes an increase in migration and invasion (Welch’s t test; p < 0.05). Box plots of all replicates within each experiment (n = 3) are depicted. MDA-MB-231-expressing GFP and MSI2-GFP were plated on transwells with and without Matrigel. 48 h after initial plating, the transwells were counted for migration c and invasion d. MSI2 increased both the migrative capabilities and invasive tendencies of MDA-MB-231 (Welch’s t test; p < 0.05). As with the MCF7, box plots of all replicates within each experiment (n = 4) are depicted. Migration e and invasion f were also assayed in MCF7 shRNA control and shRNA MSI2, respectively. The knockdown clones presented the opposite effect, with a significant decrease in the migration and invasion ability of MCF7 (Welch’s t test; p < 0.05). Proliferation assay results are summarised in Supplementary Fig. 3