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. 2017 Oct 31;7:14707. doi: 10.1038/s41598-017-14408-7

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Doxycycline improved the fatty liver in db/db mice. (A) Photos of freshly harvested livers; (B) Photo micrographs of H&E staining; White arrow: lipid droplet; Red arrow: infiltrated inflammatory cells; CV: central vein; Scale bar = 100 μm. (C) Photo micrographs of PAS staining; Black arrow: glycogenosome; Scale bar = 100 μm. Liver samples were then used for quantification of liver glycogen (D), total cholesterol (TC) (E), and triglyceride (TG) (F); Also, serum samples were used for determination of activities of alanine aminotransferase (AST) (G), and aspartate aminotransferase (ALT) (H). *p < 0.05. n = 10.