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. 2017 Sep 15;108(11):2187–2194. doi: 10.1111/cas.13380

Figure 5.

Figure 5

Exogenous expression of lipoxygenases (LOX) enhances drug‐induced ferroptosis. (a) HT1080 cells were transiently transfected with expression plasmids encoding mRFP, or mRFP‐tagged ALOX12 or ALOX15. After 24 h, red fluorescence signals were visualized by microscopy with constant fluorescence parameters. Bar, 20 μm. (b) Cells transfected with mRFP, ALOX12‐mRFP, ALOX15‐mRFP or ALOX15 with the N‐terminus deleted (ΔN)‐mRFP were treated with 1.5 μM erastin for the indicated time (upper graph). Cells transfected with mRFP or ALOX15‐mRFP were treated with 0.5 μM RSL3 for 5 h (lower graph). The cell death rate was determined by trypan blue dye‐exclusion assay. Results are the mean ± SE of three independent experiments.

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