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. 2017 Sep 14;108(11):2248–2264. doi: 10.1111/cas.13354

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© 2017 The Authors. Cancer Science published by John Wiley & Sons Australia, Ltd on behalf of Japanese Cancer Association.

This is an open access article under the terms of the Creative Commons Attribution‐NonCommercial License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.

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Solasodine induces apoptosis in colorectal cancer cells. (a) Three types of colorectal cancer cells were treated with solasodine and then analyzed by annexin V/propidium iodide (AV/PI) staining. Lower left (LL) quadrants, living cells (AV negative/PI negative). Lower right (LR) quadrants, early apoptotic cells (AV positive/PI negative). Upper right (UR) quadrants, late apoptotic cells (AV positive/PI positive). Upper left (UL) quadrants, necrotic cells (AV negative/PI positive). (b) Variations in nuclear morphology were examined by Hoechst 33258 staining and captured by fluorescence microscopy. 5‐Fu, 5‐fluorouracil.