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Solasodine regulates apoptosis‐associated genes. (a) RT–quantitative PCR analysis of apoptosis‐related mRNA. β‐Actin was selected as internal control. (b,c) Western blot analysis of apoptosis‐related proteins in all three cell lines. β‐Actin was chosen as loading control. Data are represented as the mean ± SD of three independent experiments. *P < 0.05, **P < 0.01 compared to control. 5‐Fu, 5‐fluorouracil; PARP1, poly (ADP‐ribose) polymerase 1.
