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. 2017 Oct 15;9(10):4390–4401.

Figure 7.

Figure 7

Effects of thalidomide on the expression levels and cellular activities of redox proteins. (A) The lysates of cells treated with thalidomide were analyzed by Western blotting with indicated antibodies for TrxR, GR, Trx and GPx expression. Western blot images are representative of three independent experiments. The results showed that thalidomide administration up-regulated TrxR expression dose-dependently. However, the expression levels of GR, Trx, and GPx were not affected after treating with thalidomide in both WI38VA-13 and IMR-90 cells. (B) Dose-dependent effects of thalidomide on TrxR, GR, Trx and GPx activities in WI38VA-13 and IMR-90 cells. Lysates of WI38VA-13 and IMR-90 cells treated with indicated concentrations of thalidomide for 10 h were assessed for activities of TrxR, GR, Trx and GPx. Enzyme activities were expressed as a percentage of those in DMSO-treated cells. All data points are means ± SD of two to four independent experiments. *Statistically significant difference (P < 0.05) in enzyme activity as compared to DMSO control.