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. 2017 Oct 15;9(10):4402–4414.

Figure 4.

Figure 4

MEF2D is a direct target of miR-665. A. The seed sequence of wild type (WT) 3’UTR or mutant (Mut) 3’UTR of MEF2D that is complementary to miR-665. B and C. VSMCs were co-transfected with miR-665 mimics or its control with wild type or mutant 3’UTR of MEF2D, and luciferase activity was detected at 48 h post transfection. D. The mRNA and protein expression levels of MEF2D in VSMCs was determined by qRT-PCR and western blot, respectively, at 48 h after cells transfected with miR-665 mimics or its control. MiR-665 mimics transfection suppressed the mRNA and protein levels of MEF2D. E. The VSMCs proliferation was measured by CKK-8 assay at 48 h after cells co-transfected with miR-665 mimics and pcDNA3.1-MEF2D or their respective controls. Data represents the mean values of three independent replicates ± SEM; significant differences relative to control group were shown as *P<0.05, **P<0.01, ***P<0.001.