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. 2017 Oct 15;9(10):4440–4449.

Figure 6.

Figure 6

Effects of NOX4 on JAK2/STAT3 signaling. Effects of IL-6 (100 ng/mL) on invasive capacity of MGC-803 (A) and BGC-823 cells (C) treated with NOX4 siRNA1 or control siRNA (NC) and IL-6. Phosphorylation of JAK2 and STAT3 was evaluated by immunoblotting (B, D). NC: nonsense siRNA-transfected cells; siRNA1: NOX4-siRNA1 transfected cells; NC+IL-6: nonsense siRNA and IL-6 treated cells; siRNA1+IL-6: NOX4-siRNA1 and IL-6 treated cells (*P<0.05, **P<0.01 and ***P<0.001 Vs NC; ##P<0.01 and ###P<0.001 Vs siRNA1). (E, F) SGC-7901 cells was transfected with control Vector or expression plasmid encoding NOX4. Invasion assay (E) were performed in a Matrigel-coated transwell chamber containing either DMSO or 20 μM JAK2 inhibitor, AG490. Scale bar: 100 mm. Phosphorylation of JAK2 and STAT3 was evaluated by immunoblotting (F). Vector: control plasmid-transfected cells; NOX4: NOX4 expression plasmid transfected cells; Vector+AG490: control plasmid and AG490 treated cells; NOX4+AG490: NOX4 expression plasmid and AG490 treated cells (***P<0.001 Vs Vector; ###P<0.001 Vs Vector+AG490).