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Generation of iPS cells on L-Wnt3a cell feeder layer. A. Efficiency of Oct4-GFP positive cells on L-Wnt3a cell feeder layer; B and C. Expression of pluripotent genes and epigenetic modifiers; D. Expression of transcriptional factors in iPS cells derived from L-Wnt3a cell feeder layer; E. Expression of three germ layer genes in EBs that derived from iPS cells.
