Skip to main content
. 2017 Oct 15;9(10):4694–4706.

Figure 4.

Figure 4

MiR-5100 inhibits the expression of TOB2 by binding its 3’UTR. (A) A putative miR-5100 binding site in the TOB2 3’UTR is indicated with overstriking characters. This site was identified using miRBase. (B) Luciferase reporter assay of 293T cells transfected with a psiCheck2 vector with an inserted TOB2 3’UTR sequence (TOB2 3’UTR) or a vector with an inserted mutated 3’UTR sequence (3’UTR mutant), together with either negative control miRNA (NC) or miR-5100 (mimics). The luciferase activity was normalized to firefly luciferase activity. (C) The protein levels of TOB2 was examined by Western blotting for two cell lines infected with NC, 5100-Mimic or 5100-Inhibitor. (D) Quantification of the results in panel C were carried out using Image J software. The data are expressed as the means ± SEM (n=3), (**P<0.01 versus NC).