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. 2017 Oct 11;7(10):170150. doi: 10.1098/rsob.170150

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© 2017 The Authors.

Published by the Royal Society under the terms of the Creative Commons Attribution License http://creativecommons.org/licenses/by/4.0/, which permits unrestricted use, provided the original author and source are credited.

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Figure 3. — The role of the Su(Hw) N-terminal regions in recruiting Mod(mdg4)-67.2 and CP190 to chromatin. (a) Co-immunoprecipitation between the Su(Hw) variants and Mod(mdg4)-67.2 or CP190 proteins. All designations are as in figure 2f. (b) ChIP-qPCR analysis of Su(Hw), Mod-67.2 and CP190 binding in middle pupae of transgenic lines expressing Su(Hw)+ or Su(Hw)ΔN. PCR products were amplified from three separate immunoprecipitates of three different chromatin preparations. The ras64B coding region (Ras) was used as a control devoid of Su(Hw)-binding sites. Error bars indicate the standard deviation of three independent biological replicates. *p ≤ 0.01 (Student's t-test). Other designations are as in figure 1b. The experiments were performed in the y²sc^D1ct⁶; su(Hw)^v/su(Hw)^e04061 background. Transgene abbreviations: Su(Hw)+ – P{w⁺;UbqW-Su(Hw)1-945-FLAG}/ P{w⁺;UbqW-Su(Hw)1-945-FLAG}; SuΔN – P{w⁺;UbqW-Su(Hw)238-945-FLAG}/ P{w⁺;UbqW-Su(Hw)238-945-FLAG}.