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. 2017 Oct 11;7(10):170150. doi: 10.1098/rsob.170150

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© 2017 The Authors.

Published by the Royal Society under the terms of the Creative Commons Attribution License http://creativecommons.org/licenses/by/4.0/, which permits unrestricted use, provided the original author and source are credited.

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Figure 6. — The role of the C-terminal sequences in recruiting Mod(mdg4)-67.2 to chromatin. (a) Polytene chromosomes of +/+, m^u1/ m^u1, and m^T6/ m^T6 larvae. Polytene chromosomes stained with antibodies against CP190 (red), Mod-67.2 (green), Mod-com (common part of Mod(mdg4), blue) and N-terminal region of Su(Hw) (green). Analysis of transgenic lines was performed on the y²sc^D1ct⁶; mod(mdg4)^u1/mod(mdg4)^u1 background. Arrows indicate gypsy inserts at the X-chromosome tip. (b) ChIP-qPCR analysis of Su(Hw), Mod-67.2 and CP190 binding in middle pupae of +/+, m^T6/m^T6 and m^u1/m^u1 alleles. PCR products were amplified from two separate immunoprecipitates of three different chromatin preparations. The ras64B coding region (Ras) was used as a control devoid of Su(Hw)-binding sites. Error bars indicate the standard deviation of three independent biological replicates. *p ≤ 0.05 (Student's t-test); in other cases, p ≤ 0.01. Other designations are as in figure 1b.