Fig. 4.

Neuronal knockdown of Npl4 increases TBPH accumulation. A: Confocal sections of larval ventral nerve cord (VNC) showing Venus-tagged TBPH (green) and DAPI (magenta) staining in control, Npl4 RNAi or Ufd1 RNAi neurons (generated using nSyb-GAL4). B: Graph shows quantification of the number of TBPH::Venus positive neurons throughout the VNC in each genotype. C: Knockdown of Npl4 caused increased mislocalization of TBPH to the cytoplasm compared to control (arrows). Inset (C′) shows cytoplasmic accumulations of TBPH detectable in a subset of Npl4 RNAi neurons (yellow arrowheads). D: Graph shows quantification of area of neuronal TBPH::Venus positive staining in each genotype. All data are expressed as mean ± SEM (n = 23–29 larvae from 5 independent experiments), and values significantly different from control were determined by one-way ANOVA and Dunnett's post-tests (*, P < 0.05; ns, P > 0.05).