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. 2017 Oct 20;44(10):493–501. doi: 10.1016/j.jgg.2017.06.003

Fig. 6.

Fig. 6

Knockdown of TBPH exacerbates neurodegeneration associated with knockdown of Npl4 or Ufd1. A: PCR validation of TBPH knockdown in TBPH RNAi larvae compared to control larvae. B and C: Locomotor deficits caused by neuronal specific loss of Npl4 or Ufd1 (generated using nSyb-GAL4) were exacerbated by TBPH knockdown in adult flies only. Graphs show quantification of locomotor scores in larvae (B, P values determined by one-way ANOVA and Tukey's post-tests; n = 3–10 experiments, 10 larvae per experiment; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001; ns, P > 0.05) or adult flies (C, P values determined by two-way ANOVA and Bonferroni's post-tests; n = 8–18 experiments, 10 flies per experiment). D: Loss of TBPH significantly shortened the lifespan of Npl4 knockdown flies compared to loss of either Npl4 or TBPH alone (P < 0.0001 by log-rank tests; n = 84–180 flies). Note that control, Npl4 RNAi and Ufd1 RNAi values are the same as those used in Fig. 5.