TABLE 5.
Bradyrhizobial strains and plasmids used in this study
| Strain or plasmid | Relevant characteristics and source of isolationa | Source or reference |
|---|---|---|
| Strains | ||
| PB strainsb | ||
| SUT-PR48 | Rice root isolate (rice with crop rotation) | 3 |
| SUT-PR64 | Rice root isolate (rice with crop rotation) | 3 |
| ORS285 | Aeschynomene afraspera root or stem nodule isolate | 63 |
| Non-PB strains | ||
| SUT-PR9 | Rice root isolate (rice with crop rotation) | 3 |
| SUT-R3 | Rice root isolate (rice with crop rotation) | 3 |
| SUT-R55 | Rice root isolate (rice with crop rotation) | 3 |
| SUT-R74 | Rice root isolate/paddy soil isolate (rice with crop rotation) | 3 |
| SUTN9-2 | Aeschynomene americana nodule isolate (paddy crop) | 62 |
| SUTN9-2GUS | SUTN9-2 marked with mTn5SSgusA20 (pCAM120); Smr Spr | 54 |
| SUTN9-2nifH-GUS | Transcriptional nifH::gus reporter construct (pVO155nifHpm9-2gus) | This work |
| PRC008 | Recommended for mung bean/non-rice endophyte | Department of Agriculture (DOA), Thailand |
| Escherichia coli S17-1 | pro recA RP4-2 (Tcs::Mu) (Kms::Tn7), Mob+ | 64 |
| Plasmids | ||
| pCAM120 | mTn5SSgusA20 in pUT/mini-Tn5 | S. Okazakic; 65 |
| pVO155nifHpm9-2gus | nifH::gus reporter construct into pVO155-npt2-cefo-npt2-gfp; Kmr Cefor | This work |
a
Smr, spectinomycin resistance; Spr, streptomycin resistance; Tcs, tetracycline susceptibility; Kms, kanamycin susceptibility; Kmr, kanamycin resistance; Cefor, cefotaxime resistance.
b
PB, photosynthetic Bradyrhizobium.
c
Department of International Environmental and Agricultural Science, Graduate School of Agriculture, Tokyo University of Agriculture and Technology, Tokyo, Japan.