FIG 2.

Growth curves in LB medium and LB medium with 200 μM NaHS. Overnight cultures were inoculated into LB medium or LB medium containing 200 μM NaHS, at an initial OD₆₀₀ of 0.05. Cell turbidity was assessed every 2 h. ■, C. necator H16 in LB medium; ●, C. necator H16 in LB medium with 200 μM NaHS; □, C. necator H16 ΔfccAB in LB medium; ○, C. necator H16 ΔfccAB in LB medium with 200 μM NaHS; ▲, C. necator H16 ΔfccAB/fccAB in LB medium; △, C. necator H16 ΔfccAB/fccAB in LB medium with 200 μM NaHS. All data are averages of three samples with standard deviations (error bars). All cultures reached final OD₆₀₀ values of about 6.1 after 24 h of incubation.