TABLE 3.
Strain or plasmid | Genotype and/or descriptiona | Source or reference |
---|---|---|
Strain | ||
E. coli DH5α | Cloning strain | Novagen |
E. coli S17-1 | RP4 derivative integrated in chromosome | 56 |
E. coli BL21(DE3) | Cloning strain | Novagen |
C. pinatubonensis JMP134 | Wild type | Ron L. Crawford |
C. necator H16 | Wild type | ATCC 17699 |
P. aeruginosa PAO1 | Wild type | ATCC 15692 |
Pa3K | Mutant of P. aeruginosa PAO1 in which sqr1, sqr2, and pdo were deleted | This study |
Plasmid | ||
pBBR1MCS-3 | Tetr, Plac; expression vector | 57 |
pBBR1MCS-5 | Gmr, Plac; expression vector | 57 |
pK18mobsacBtet | Kmr and Tetr, sacB, RP4 oriT, ColE1 ori; suicide vector | This study |
pBBR5-fccAB | pBBR1MCS-5 containing fccAB from C. necator H16 | This study |
pBBR5-fccAB-pdo1 | pBBR1MCS-5 containing fccAB and pdo1 from C. necator H16 | This study |
pBBR5-fccAB-pdo2 | pBBR1MCS-5 containing fccAB and pdo2 from C. necator H16 | This study |
pBBR3-fccAB | pBBR1MCS-3 containing fccAB from C. necator H16 | This study |
pBBR3-fccAB-pdo1 | pBBR1MCS-3 containing fccAB and pdo1 from C. necator H16 | This study |
pBBR3-fccAB-pdo2 | pBBR1MCS-3 containing fccAB and pdo2 from C. necator H16 | This study |
pK18mobsacBtet-ΔfccAB | Tetr; suicide vector for deletion of fccAB of C. necator H16 | This study |
Tetr, tetracycline resistance; Gmr, gentamycin resistance; Kmr, kanamycin resistance.