TABLE 3.
Bacteria and plasmids used in this study
| Strain or plasmid | Genotype and/or descriptiona | Source or reference |
|---|---|---|
| Strain | ||
| E. coli DH5α | Cloning strain | Novagen |
| E. coli S17-1 | RP4 derivative integrated in chromosome | 56 |
| E. coli BL21(DE3) | Cloning strain | Novagen |
| C. pinatubonensis JMP134 | Wild type | Ron L. Crawford |
| C. necator H16 | Wild type | ATCC 17699 |
| P. aeruginosa PAO1 | Wild type | ATCC 15692 |
| Pa3K | Mutant of P. aeruginosa PAO1 in which sqr1, sqr2, and pdo were deleted | This study |
| Plasmid | ||
| pBBR1MCS-3 | Tetr, Plac; expression vector | 57 |
| pBBR1MCS-5 | Gmr, Plac; expression vector | 57 |
| pK18mobsacBtet | Kmr and Tetr, sacB, RP4 oriT, ColE1 ori; suicide vector | This study |
| pBBR5-fccAB | pBBR1MCS-5 containing fccAB from C. necator H16 | This study |
| pBBR5-fccAB-pdo1 | pBBR1MCS-5 containing fccAB and pdo1 from C. necator H16 | This study |
| pBBR5-fccAB-pdo2 | pBBR1MCS-5 containing fccAB and pdo2 from C. necator H16 | This study |
| pBBR3-fccAB | pBBR1MCS-3 containing fccAB from C. necator H16 | This study |
| pBBR3-fccAB-pdo1 | pBBR1MCS-3 containing fccAB and pdo1 from C. necator H16 | This study |
| pBBR3-fccAB-pdo2 | pBBR1MCS-3 containing fccAB and pdo2 from C. necator H16 | This study |
| pK18mobsacBtet-ΔfccAB | Tetr; suicide vector for deletion of fccAB of C. necator H16 | This study |
a
Tetr, tetracycline resistance; Gmr, gentamycin resistance; Kmr, kanamycin resistance.