TABLE 5.
Sources of DNA for qPCR standard curve generation
| ARG | Strain or plasmid | Source |
|---|---|---|
| aac(6′)-Ie-aph(2″)-Ia | Enterococcus faecalis ATCC 51299 | ATCCa |
| aadA1 | pSkunk3-BLA | Addgene, Cambridge, MA |
| blaCMY-2 | Escherichia coli J79 | Schmidt laboratory collection, beef cattle isolate |
| blaCTX-M | E. coli J82 | Schmidt laboratory collection, beef cattle isolate |
| blaKPC-2 | Klebsiella pneumoniae (Schroeter) Trevisan ATCC BAA-1898 | ATCC |
| erm(B) | E. coli 52185 | Gift from Dayna Harhay, USMARC |
| mecA | Staphylococcus aureus ATCC 43300 | ATCC |
| tet(A) | E. coli J106 | Schmidt laboratory collection, beef cattle isolate |
| tet(B) | E. coli J108 | Schmidt laboratory collection, beef cattle isolate |
| tet(M) | Enterococcus sp. J78 | Schmidt laboratory collection, beef cattle isolate |
a
ATCC, American Type Culture Collection.