FIG 3 .

C. neoformans cells expressing low Atm1 protein levels are more sensitive to Cu stress than wild-type cells. (A) Scheme representing steps for the experimental procedures performed as described for panel B. Cultures of C. neoformans strains Atm1-F (DTY947) and Gal7-Atm1-F (DTY949) were diluted in either galactose-containing media or glucose-containing media for 3 days and diluted into 96-well plates to an OD of 0.002 in galactose-containing media or glucose-containing media, respectively, with the indicated concentrations of Cu. The final OD was recorded after 48 h. (B) Cultures of C. neoformans strains prepared as shown in panel A were grown in SC-Gal media (left panel) or in SC-Gluc media (right panel) with the indicated amounts of Cu. n = 3 (3-way repeated-measures ANOVA test). Cu differentially impacted growth of the strains only in the presence of glucose (P < 0.0001).