Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2017 Oct 30;8:1398. doi: 10.3389/fimmu.2017.01398

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2017 Bittner-Eddy, Fischer, Tu, Allman and Costalonga.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

PMC Copyright notice

Intravenous anti-CD45 mAb distinguishes interstitial leukocytes from circulating leukocytes in oral mucosa. Anesthetized C57BL/6J mice were given 1.25 µg of FITC-conjugated anti-CD45 (CD45:FITC) mAb in 200 µl of PBS via retro-orbital intravenous injection. Mice were euthanized at 3 min. Single-cell suspensions obtained from blood, cervical lymph nodes (CLNs), and oral mucosa were stained with vitality dye Zombie NIR followed by rat anti-Mouse CD45 mAb conjugated to PE (CD45:PE). Cells were analyzed by flow cytometry. Dot plots from (A) blood, (B) CLN, and (C) oral mucosa samples gated on live cells obtained from a representative mouse are shown.