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. 2017 Sep 25;9(11):1471–1481. doi: 10.15252/emmm.201707795

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© 2017 The Authors. Published under the terms of the CC BY 4.0 license

This is an open access article under the terms of the Creative Commons Attribution 4.0 License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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Figure EV3 — DNA genomic fragments encompassing C/T₁₃₉₁₀ and G/A₂₂₀₁₈ nucleotides were amplified and digested as described in Matthews et al (2005). The digested PCR products were analyzed on agarose gel. Lanes 1 and 8 show molecular weight markers. Lanes 2 and 9 show non‐digested (ND) PCR products. Lanes 3 and 10 show the CC₁₃₉₁₀ and GG₂₂₀₁₈ homozygous lactose intolerance genotypes, respectively. Lanes 4 and 11 show the TT₁₃₉₁₀ and AA₂₂₀₁₈ homozygous lactase persistent genotypes, respectively. Lanes 5 and 12 show the CT₁₃₉₁₀ and GA₂₂₀₁₈ heterozygous lactase persistent genotypes, respectively. Lanes 6 and 13 show non‐digested PCR products from Caco‐2 cells. Lanes 7 and 14 show digested PCR products displaying LCT genotyping polymorphisms C/T₁₃₉₁₀ and G/A₂₂₀₁₈ for Caco‐2 cells. Migration profile reveals that Caco‐2 cells possess the CC₁₃₉₁₀ (lane 7) and GG₂₂₀₁₈ (lane 14) homozygous lactose intolerance genotypes.