Skip to main content
. 2017 Sep 19;9(11):1589–1604. doi: 10.15252/emmm.201707814

Figure 3. The glycolytic profile of HB cell lines is correlated to β‐catenin mutation.

Figure 3

  1. Real‐time PCR using specific probes for glycolytic (HK1, PFKP, LDHB, HK2) and gluconeogenesis (AQP9, GK, G6PC, PPARGC1A) genes. Data show means ± s.d. (n = 3). P‐values were determined by Mann–Whitney test.
  2. Cytoplasmic and nuclear protein extracts isolated from the indicated cell lines were analyzed for β‐catenin expression by Western blot.
  3. Histograms of the RNAseq read counts for the CTNNB1 region in the embryonal and fetal cell lines.
  4. Real‐time PCR on GLUT3 after siGLUT3 or siCtrl transfection. Data show means ± s.d. (n = 3). P‐values were determined by Mann–Whitney test.
  5. Glucose uptake assay in HepG2 and Huh‐6 after transfection with siGLUT3 or siCtrl. Data show means ± s.d. (n = 5). P‐values were determined by Mann–Whitney test.
  6. Seahorse glycolytic assay on the three hepatoblastoma cell lines after transfection with siGLUT3 or siCtrl. Sequential measurements of ECAR from 10 replicates after the injections were done. Data show means ± s.d. Multiple t‐test was used.

Source data are available online for this figure.