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. 2017 Nov 2;4(5):ENEURO.0133-17.2017. doi: 10.1523/ENEURO.0133-17.2017

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Copyright © 2017 Mohr et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license, which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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Figure 1. — Pubertally born cells were activated by estradiol and P treatment. A, Representative images of a pubertally born cell that was also Fos-ir after a hormone-induced LH surge in adulthood. Images are a maximum intensity projection of a Z-stack (0.5-μm step) captured using the MBF Bioscience Image Stack Module. Scale bar, 10 μm. An orthogonal view (right) through the middle of the cell confirms colocalization; red arrow, x plane; green arrow, y plane; and blue arrow, z plane. B, Hormone induction of the LH surge caused a significant increase in the percentage of pubertally born BrdU-ir cells that express Fos-ir (*p ≤ 0.0001). The graph represents mean ± SEM; for oil treated, n = 6, for hormone treated, n = 7.