Figure 2.

Post-translational modifications (PTMs) observed for Bp-Ac-Hly ((A) PTMs on K-860; (B) PTMs on K-983). From the modifications Specific Peptides output table of the MaxQuant results, intensities of the unmodified and modified peptides of interest (peptide forms) were extracted. For each lysine residue (K-860 and K-983), intensities were summed by peptide form and normalized against the sum of all extracted intensities per involved lysine. These calculated percentages were plotted on the bar chart representation: Myristoylation (light grey), Palmitoyalation (intermediate grey), no PTMs (dark). The intensities of peptide ions used in the bar charts (Figure 2 and Figure 3) do not necessarily directly correlate with the actual amount of each corresponding peptide since ionization efficiencies can vary with the presence of post-translational modifications. However, myristoylation and palmitoylation are comparable modifications that occur on the same amino acid (Lysine), and, thus, we can postulate that the ionization efficiencies of the corresponding peptides should not be very different.