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. 2017 Oct 12;9(10):315. doi: 10.3390/toxins9100315

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© 2017 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Analysis of hbx1 and developmental gene expression in AF70 strains. (A) RT-qPCR of hbx1 expression in the AF70 pyrG-1 control strain at 6, 24 and 48 h time points. (B) Analysis of hbx1 and developmental gene expression in the AF70 Δhbx1 #4 (white bars) and Δhbx1-com #8 strains (black bars). Expression is relative to a level of 1 set for the AF70 control. Cultures were grown and samples prepared as described in Materials and Methods. The relative gene expression levels for all time points were normalized to the A. flavus 18S rRNA C_T values as part of the ΔΔC_T analysis method. A Fold-Change formula (2^−ΔΔC_T) [44] was used in the conversion of the ΔΔC_T results for a final calculation of values.