Figure 2.

(a) Fluorescence quenching of 5′-FAM-labeled AFM₁ aptamer after incubated with different concentrations of 33 nm PdNPs (0, 0.015 mg/mL, 0.030 mg/mL, 0.045 mg/mL, 0.060 mg/mL, 0.075 mg/mL, 0.090 mg/mL). (b) Fluorescence quenching of fluorescein in the presence of 0.060 mg/mL PdNPs (33 nm). (c) Time dependence of the fluorescence quenching degree for 80 nM 5′-FAM-labeled AFM₁ aptamer caused by 0.060 mg/mL PdNPs (33 nm). All experiments were performed in HEPES buffer under excitation at 480 nm and emission at 520 nm.