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. 2017 Oct 13;9(10):320. doi: 10.3390/toxins9100320

Figure 1.

Figure 1

SDS-PAGE analysis of heterogeneous toxin complexes and subunits. An amount of 100 ng per lane of sample (abrin, abrin A-chain, abrin B-chain) treated or not treated with 0.05 M DTT was loaded onto a NuPAGE 4–12% Bis-Tris gel and subjected to SDS-PAGE electrophoresis. The gel was silver stained with the SilverXpress kit. In the absence of the reducing agent, abrin predominantly consists of the holotoxin with small amounts of agglutinin, A-chain, and B-chain. Once reduced with DTT, abrin is almost all reduced to the smaller individual A- and B-chains. The abrin A-chain control sample with DTT has two predominant A-chain species. The reduced abrin B-chain control has one predominant species that is of higher molecular weight than the individual A-chain control.