Figure 2.

Translation inhibition by the plant toxin Abrin was abrogated by exposure to high temperature. (A) A representative standard curve shows increasing translation inhibition until saturation as one increased the abrin concentration in the in vitro cell free translation (CFT) assay run in parallel with the experimental samples shown in (B). Values represent means of triplicate samples ± SD. (B) Cell free translation assay using a single concentration of toxin (100 ng/mL) for all the different conditions. Increasing the temperature that abrin is exposed to decreases the translational inhibition seen in the CFT assay. Values represent means of triplicate samples ± SD. Statistical significance was determined by two-tailed unpaired Student’s t-test, (****) p < 0.0001. Two independent experiments in triplicate were performed and one representative data set is presented.