Table 1.
In vitro Vero cell cytotoxicity assay of temperature-treated toxins.
| Treatment | Relative Cytotoxicity (%) |
|---|---|
| DMEM | 0 |
| Abrin | 100 |
| 99 °C | 0 |
| 85 °C | 0 |
| 80 °C | 0 |
| 74 °C | 0 |
| 63 °C | 72 ± 4 |
Vero cell cytotoxicity after treatment with abrin, either treated or not with increasing temperatures. Abrin was used at 5 ng/mL for these assays. Values represent means of six samples ± SD. Statistical significance was determined by two-tailed unpaired Student’s t-test, p < 0.0001 for all conditions compared with abrin non-treated. Two independent experiments were performed and one representative data set is presented.