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. 2017 Oct 13;9(10):321. doi: 10.3390/toxins9100321

Figure 5.

Figure 5

Effect of protease inhibitors on antimicrobial activities of the cell-free supernatant including MP-V1 in increased Salmonella population density. (A) Examination of antimicrobial activity of the cell-free supernatant including MP-V1 according to the Salmonella population density. The antimicrobial activity of 100 μL cell-free supernatant from the OmpA SS::MP-V1 strain was examined against 103 to 108 cfu/mL of Salmonella Typhimurium. (B,C) The effect of the protease inhibitor cocktail and EDTA on antimicrobial activities of the cell-free supernatant including MP-V1 in increased Salmonella population density. The effect of a protease inhibitor cocktail (B) and EDTA (C) on antimicrobial activities of 100 μL cell-free supernatant from the OmpA SS::MP-V1 strain was examined against 108 cfu/mL of Salmonella Typhimurium using 0.5 to 10 μL doses and 1 to 25 mM doses, respectively. Data are means ± SE (n = 3). Different letters indicate significant differences by the one-way ANOVA/Duncan (p < 0.05).