Multiple alignment and identity matrix of pilosulin-related peptide amino acid sequences. Percentage amino acid sequence identities between melittin (NP_001011607.1), pilosulin 1 (Q07932.1), and pilosulin-like peptides (PLPs) are shown in (A). The amino acid sequences of melittin and pilosulin-like peptides were aligned with ClustalW in Lasergene 12 (DNASTAR, Madison, WI, USA). Arrows indicate the putative processing and modification sites for signal peptidase, dipeptidyl peptidase, amidating lyase, and carboxypeptidase. Proline and alanine residues in the spacer region between the signal- and mature peptides of pilosulin-related peptides are highlighted in yellow. Nucleotide sequences for PLP 1, 2, 3, 4, 5, and 6 were assigned DDBJ/EMBL/GenBank Accession Numbers LC316119, and FX985495-9, respectively (B).