Figure 7.
Effect of antitoxin on uptake and trafficking of ricin in human cultured A549 cells. (A) Antitoxin reduces cellular uptake and appearance of ricin within the cellular cytoplasmic mask in a concentration dependent manner. (B) In those cells that are positive for cytoplasmic ricin, antitoxin reduces the percentage of cells that have Golgi-associated ricin compared to control. Data in (A,B) are shown as mean ± SD from three independent experiments. * p < 0.05 (two-way ANOVA with multiple comparisons). (C,D) Example images of F(ab’)2 and ricin interactions at 15 and 60 min as shown as part of the ImageStreamX analysis (BF = Brightfield).