Figure 1.
Detection of recombinant Clostridium perfringens β-toxin (rCPB)-induced rapid cell death in porcine platelets by flow cytometry. Platelets (33.3 × 106/mL) were incubated with rCPB (10 and 250 ng/mL) or neutralized rCPB for indicated time periods. Viability staining was performed using Calcein-AM (1 μM). Platelets in medium without toxin served as negative control. The histograms show the Calcein-AM fluorescence intensity of platelets, in which the Calcein-AM positive fraction represents viable platelets. Percentages of platelets are indicated in the top of each panel.