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Porcine platelets exposed to rCPB are irresponsive to thrombin activation. Platelets were incubated with PBS (control) or rCPB for the indicated times followed by addition of thrombin. While control platelets reacted to thrombin with typical shape change and formation of pseudopodia at all time points, platelets incubated with rCPB (250 ng/mL) did not display such thrombin-induced changes.
