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. 2017 Oct 19;7(11):1815–1825. doi: 10.1002/2211-5463.12328

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© 2017 The Authors. Published by FEBS Press and John Wiley & Sons Ltd.

This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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FRET assays for GagPol homodimerization with interdomain constructs. HeLa cells were cotransfected with GagPol(MA/F/CA), GagPol(p6*/F/PR), or GagPol(PR/F/RT) molecular clone pair supplemented with authentic Gag‐expressing construct (donor GagPol: acceptor GagPol: Gag = 1 : 1 : 2). Combinations of GagPol(MA/EGFP/CA), GagPol(p6*/EGFP/PR), or GagPol(PR/F/RT) molecular clone and a plasmid expressing mSB were used as negative controls. The FRET efficiencies were statistically greater than those of the corresponding negative controls (P < 0.01). *Statistically significant (P < 0.01) as compared with the GagPol(MA/F/CA) pair. Representative FRET images are shown.