Figure 6.

Nitric oxide (NO) bioavailability and iNOS expression are increased following AGE treatment. (A) Representative images of control and AGE‐treated NRCM stained with NO‐sensitive dye DAF‐FM. (B) Quantification of DAF‐FM fluorescent signal revealed a significantly higher NO bioavailability in AGE‐treated NRCM compared to control (P < 0.05, n = 94–106 cells). (C) Representative western blots and (D) quantification of band density showing expression of iNOS in control and AGE‐treated NRCM (P < 0.01, n = 7 in each group).