Table 3.
Strains and plasmids used in this study.
| Name | Description | Reference/Sources |
|---|---|---|
| Strain | ||
| E. coli BETA-1 | β-Carotene producing strain | [18] |
| E. coli ZEAX | Zeaxanthin producing strain, one copy of Pantoea ananatis crtZ under the control of the P37 promoter was integrated into E. coli BETA-1 chromosome | [19] |
| E. coli ZEAX-4 | Zeaxanthin producing strain, two copies of P. ananatis crtZ under the control of the P37 promoter was integrated into E. coli BETA-1 chromosome | This study |
| E. coli ASTA | Astaxanthin producer, one of B. sp. SD212 crtW under the control of the P37 promoter was integrated into E. coli ZEAX-4 chromosome | This study |
| E. coli ASTA-1 | Astaxanthin producer, another copy of P. ananatis crtY under the control of the P37 promoter was integrated into E. coli ASTA-1 chromosome | This study |
| Plasmid | ||
| pZSABP | Constitute expression vector, pSC101 ori, P37 promoter, Ampr, BglBrick, ePathBrick containing four isocaudamer (AvrII, NheI, SpeI and XbaI) | [18] |
| pBAD33 | Expression vector, PBAD, p15A ori, Cmr | [24] |
| pZS-crtWBsp | pZSABP containing Brevundimonas sp. SD212 crtW under the control of the P37 promoter | This study |
| pZS-crtWPsp | pZSABP containing Paracoccus sp. N81106 crtWL175W under the control of the P37 promoter | This study |
| pZS-crtWSsp | pZSABP containing Sphingomonas sp. DC18 crtWF213L/R203W under the control of the P37 promoter | This study |
| pZS-bkt | pZSABP containing Chlamydomonas reinhardtii bkt under the control of the P37 promoter | This study |
| pZS-2crtWBsp | pZSABP containing two copies of B.s sp. SD212 crtW under the control of the P37 promoter | This study |
| pZS-2bkt | pZSABP containing two copies of C. reinhardtii bkt under the control of the P37 promoter | This study |
| pZS-2crtWPsp | pZSABP containing two copies of P. sp. N81106 crtWL175W under the control of the P37 promoter | This study |
| pZS-2crtWSsp | pZSABP containing two copies of S. sp. DC18 crtWF213L/R203W under the control of the P37 promoter | This study |
| pZS-crtWBspcrtWPsp | pZSABP containing B.s sp. SD212 crtW under the control of the P37 promoter and P. sp. N81106 crtWL175W under the control of the P37 promoter | This study |
| pZS-crtWBspcrtWSsp | pZSABP containing B.s sp. SD212 crtW under the control of the P37 promoter and S. sp. DC18 crtWF213L/R203W under the control of the P37 promoter | This study |
| pZS-crtWBspbkt | pZSABP containing B.s sp. SD212 crtW under the control of the P37 promoter and C. reinhardtii bkt under the control of the P37 promoter | This study |
| pZS-crtWPspcrtWSsp | pZSABP containing P. sp. N81106 crtWL175W under the control of the P37 promoter and S. sp. DC18 crtWF213L/R203W under the control of the P37 promoter | This study |
| pZS-crtWPspbkt | pZSABP containing P. sp. N81106 crtWL175W under the control of the P37 promoter and C. reinhardtii bkt under the control of the P37 promoter | This study |
| pZS-crtWSspbkt | pZSABP containing S. sp. DC18 crtWF213L/R203W under the control of the P37 promoter and C. reinhardtii bkt under the control of the P37 promoter | This study |
| pBAD-crtZ | pBAD33 containing P. ananatis crtZ | This study |
| pBAD-crtWBsp | pBAD33 containing B.s sp. SD212 crtW | This study |