Table 2.
Amino acid profiles of F. spiralis protein hydrolysate fractions (mg amino acids/g of DW FSPH) obtained by ultrafiltration.
| Amino Acid (AA) | F. spiralis Protein Hydrolysate (FSPH) Fraction | ||
|---|---|---|---|
| Fr1 < 1 kDa | 1 kDa ≤ Fr2 < 3 kDa | Fr3 ≥ 3 kDa | |
| Alanine | tc | tc | 25.34 ± 0.13 |
| Glycine | tc | tc | 22.38 ± 0.13 |
| Valine | 155.53 ± 2.25 | 209.37 ± 3.35 | 236.83 ± 4.65 |
| Threonine | 1.22 ± 0.08 | 1.71 ± 0.03 | 17.42 ± 0.13 |
| Serine | 2.75 ± 0.06 | 3.18 ± 0.03 | 19.36 ± 0.16 |
| Leucine | 2.24 ± 0.12 | 2.85 ± 0.05 | 25.35 ± 0.17 |
| Isoleucine | 1.89 ± 0.03 | 2.84 ± 0.06 | 32.55 ± 0.43 |
| Proline | 1.34 ± 0.05 | 1.93 ± 0.03 | 12.47 ± 0.11 |
| Methionine | tc | tc | 20.07 ± 0.31 |
| Aspartic acid | 3.63 ± 0.07 | 4.53 ± 0.10 | 37.53 ± 0.35 |
| Phenylalanine | 1.80 ± 0.02 | 1.49 ± 0.02 | 15.13 ± 0.15 |
| Glutamic acid | 10.40 ± 0.10 | 7.01 ± 0.22 | 46.33 ± 0.42 |
| Lysine | 15.48 ± 0.20 | 7.57 ± 0.11 | 13.82 ± 0.13 |
| Tyrosine | tc | tc | 38.17 ± 0.21 |
| Arginine | tc | tc | tc |
| Histidine | tc | tc | tc |
| Tryptophan | ND | ND | ND |
| Total AA | 196.28 a | 242.48 a | 562.75 b |
| AA distribution (%) | |||
| Hydrophobic | 82.94 a | 90.10 a | 65.35 b |
| Hydrophilic | 15. 04 a | 7.88 b | 17.36 a |
| Neutral | 2.02 b | 2.02 b | 17.29 a |
| Aromatic | 0.92 b | 0.61 b | 9.47 a |
| Branched-side chains | 81.34 a | 88.69 a | 52.37 b |
| Negatively charged | 7.15 b | 4.76 c | 14.90 a |
| Positively charged | 7.89 a | 3.12 b | 2.46 b |
Values are mean ± SD (n = 3). Means with different superscripts letters in the same row differ significantly (p < 0.05). Hydrophobic AA (Ala, Val, Leu, Ile, Pro, Met, Phe). Hydrophilic AA (Asp, Glu, Lys, Arg, His). Neutral AA (Gly, Thr, Ser, Tyr). Aromatic AA (Phe, Tyr). Branched-side chains AA (Val, Leu, Ile). Negatively charged AA (Asp, Glu). Positively charged AA (Lys, Arg, His). Tryptophan is not detected in this methodology. DW, dry weight; ND, not detected; tc, traces.