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. 2017 Oct 16;15(10):314. doi: 10.3390/md15100314

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© 2017 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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The mitochondrial reactive oxygen species (ROS) level assay. The effect of C1 sub-fraction of methanolic extract of H. parva (250, 500 and 1000 μg/mL) on mitochondrial ROS generation in: the normal mitochondria (A); and the HCC mitochondria (B) obtained from liver hepatocytes within 60 min of incubation. Mitochondrial Swelling assay. The effect of C1 sub-fraction of methanolic extract of H. parva (250, 500 and 1000 μg/mL) on mitochondrial swelling in: the normal mitochondria (C); and the HCC mitochondria (D) obtained from liver hepatocytes within 60 min of incubation. Data are shown as mean ± SD (n = 3). (A,C) Control means vehicle treated normal mitochondria. (B,D) Control means vehicle treated HCC mitochondria. **, *** and **** show significant differences in comparison with corresponding control (HCC) groups for p < 0.01, p < 0.001 and p < 0.0001, respectively.