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. 2017 Oct 16;15(10):314. doi: 10.3390/md15100314

Figure 7.

Figure 7

The Mitochondrial Membrane Potential (MMP) assay. The effect of (Z)-2,3-diphenylacrylonitrile of H. parva (10, 20 and 40 μg/mL) on MMP collapse in: the normal mitochondria (A); and the HCC mitochondria (B) obtained from liver hepatocytes at within 60 min of incubation. (A) Control means vehicle treated normal mitochondria; (B) Control means vehicle treated HCC mitochondria; (C) The cytochrome c release assay. The effect of (Z)-2,3-diphenylacrylonitrile of H. parva (20 μg/mL) on cytochrome c release in the normal mitochondria and the HCC mitochondria obtained from liver hepatocytes at within 60 min of incubation; (D) MTT assay: Hepatocytes obtained from HCC group were treated with (Z)-2,3-diphenylacrylonitrile of H. parva (0, 2.5, 5, 10, 20, 40 and 100 μg/mL) and incubated for 24 h. Data are shown as mean ± SD (n = 3). *, **, *** and **** indicate significant differences in comparison with corresponding control (HCC) groups for p < 0.001 and p < 0.0001, respectively. ### Significant differences in the comparison with 20 μg/mL (HCC) (Z)-2,3-diphenylacrylonitrile (p < 0.001).