. 2017 Oct 5;33(43):12443–12452. doi: 10.1021/acs.langmuir.7b02931

Table 1. Overview of the Synthesized Derivatives of Peptides E₃ and K₃ Used in This Study^a.

spacer	DOPE anchor	L_PEG^d	cholesterol anchor	L_PEG^d
none^b	LP₀E₃/LP₀K₃	0.5	CP₀E₃/CP₀K₃	–0.5
PEG₂^c	LP₂E₃/LP₂K₃	1.6	n.a.	n.a.
PEG₄	LP₄E₃/LP₄K₃	2.3	CP₄E₃/CP₄K₃	1.3
PEG₈	LP₈E₃/LP₈K₃	3.9	CP₈E₃/CP₈K₃	2.9
PEG₁₂	LP₁₂E₃/LP₁₂K₃	5.4	CP₁₂E₃/CP₁₂K₃	4.4
PEG₁₆^c	n.a.	n.a.	CP₁₆E₃/CP₁₆K₃	5.8

Peptide purities of >95% were confirmed using LC-MS for all constructs.

The peptide is connected to the anchor via a succinic diamide, with a length of 0.5 nm.

An LP₂ spacer and a CP₁₆ spacer were added to the PEG series to cover a significant range close to the maximum fusion efficiency.

L_PEG is the distance between the N-terminus of the peptide and the surface of the membrane in nanometers. For DOPE anchors, this is the maximum theoretical chain length of the PEG spacer (nm), as estimated by AVOGADRO,⁴³ and for cholesterol anchors, this is the maximum theoretical chain length, 1.0 nm,⁴⁴ due to the intrinsic membrane insertion property of cholesterol in DOPC/DOPE membranes.

Table 1. Overview of the Synthesized Derivatives of Peptides E3 and K3 Used in This Studya.

Table 1. Overview of the Synthesized Derivatives of Peptides E₃ and K₃ Used in This Study^a.