. 2017 Oct 5;33(43):12443–12452. doi: 10.1021/acs.langmuir.7b02931

Table 3. Multiple Rounds of Fusion Assay.

functionalized liposomes	CP₈K₃ +			CP₁₂K₃ +			CP₁₆K₃ +
[CP_nK₃]/[CP_mE₃]^a	CP₈E₃	CP₁₂E₃	CP₁₆E₃	CP₈E₃	CP₁₂E₃	CP₁₆E₃	CP₈E₃	CP₁₂E₃	CP₁₆E₃
1^b	37	38	33	37	47	29	25	47	29
10	41	41	36	76	111	59	40	72	42

Δ%^c	10%	7%	8%	104%	135%	104%	61%	53%	46%

Fusion efficiency is indicated by an increase in sulforhodamine B emission after 30 min, with K₃-decorated liposomes in 1- or 10-fold excess with respect to dye-loaded, E₃-decorated liposomes.

Fusion efficiency percentages are normalized to efficiency percentages reported for the equimolar mixture at a total lipid concentration of 0.1 mmol, as shown in Figure 4.

Differences in the fluorescence increase between both experiments are calculated as a measure of multiple rounds of fusion, and the value of the normalized equimolar mixing entry used is 100%. [Total lipid E₃-decorated liposomes] = 0.05 mM, with 1 mol % lipopeptides, PBS pH 7.4.