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. 2017 Sep 4;14(11):1101–1109. doi: 10.7150/ijms.20104

Figure 3.

Figure 3

Induction of apoptosis in G361 cells by p-FAK-GNP. (A) The G361 cells were treated with p-FAK-GNP for 12 to 48 h. At each time point, the cells were subjected to FACS analysis using PI solution as indicated. (B) After a 24 h incubation in the presence (lower panel) or absence (upper panel) of p-FAK-GNP, the translocation of cytochrome c was visualized by immunocytochemistry using an anti-cytochrome c antibody. (C) After 24 h incubation in the presence (lower panel) or absence (upper panel) of p-FAK-GNPs, the subcellular localization of AIF was visualized by immunocytochemistry using an anti-AIF antibody. (D) The G361 cells were incubated in the presence of p-FAK-GNP for the indicated times. The total protein lysates were used for SDS-PAGE followed by western blots using antibodies against BAX, Bdl-2, Caspase 3, Caspase 9, PARP-1, DFF 45, and GAPDH (as a loading control).