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. 2017 Sep 30;14(12):1284–1291. doi: 10.7150/ijms.20396

Figure 3.

Figure 3

TSN reduced AngII-induced apoptosis and promoted survival via estrogen receptors in H9c2 cardiocytes. (A) Twenty-four hours after seeding the H9c2 cells in 10-cm culture plates, 10-8 M AngII was added, followed by 40μM TSN and 10-6 M ICI. Western blot analysis was conducted 24 h after the administration of ICI. (B) Fold change were calculated and are shown. (C) Twenty-four hours after seeding the H9c2 cells in 10-cm culture plates, 10-8M AngII was added, followed by 40μM TSN and 10-6 M ICI. TUNEL staining was conducted 24 h after the administration of ICI (400X magnification). Data are expressed as the fold change relative to the control and presented as the mean ± SD, representing the results of three independent experiments (n = 3, *p < 0.05 was considered significant).