Table 1.
Resuming currents and spiking probability of Chronos-expressing neurons photoactivated by illuminating the cellular soma with circular 2P holographic spots
| Interneurons | Pyramidals | |||||||
|---|---|---|---|---|---|---|---|---|
| Current | 415 ± 213 pA (n = 38) | 368 ± 172 pA (n = 12) | ||||||
| Latency | 2.83 ± 0.97 ms (n = 24) | 6.4 ± 2.6 ms (n = 6) | ||||||
| Jitter | 0.19 ± 0.16 ms (n = 24) | 0.3 ± 0.2 ms (n = 6) | ||||||
| AP probability p < 0.12 mW/μm2 | 28/38 | 5/12 | ||||||
| AP probability p < 0.30 mW/μm2 | 32/38 | 8/12 | ||||||
| 10 Hz firing probability | AP1 (n = 11) | AP2 (n = 11) | AP3 (n = 11) | AP4 (n = 11) | AP1 (n = 3) | AP2 (n = 3) | AP3 (n = 3) | AP4 (n = 3) |
| 1 ± 0 | 1 ± 0 | 1 ± 0 | 1 ± 0 | 1 ± 0 | 1 ± 0 | 1 ± 0 | 0.66 ± 0.57 | |
| 25 Hz firing probability | — | AP1 (n = 2) | AP2 (n = 2) | AP3 (n = 2) | AP4 (n = 2) | |||
| 1 ± 0 | 1 ± 0 | 1 ± 0 | 0.66 ± 0.57 | |||||
| 50 Hz firing probability | AP1 (n = 10) | AP2 (n = 10) | AP3 (n = 10) | AP4 (n = 10) | AP1 (n = 2) | AP2 (n = 2) | AP3 (n = 2) | AP4 (n = 2) |
| 1 ± 0 | 1 ± 0 | 1 ± 0 | 1 ± 0 | 0.83 ± 0.24 | 0.5 ± 0.7 | 0.83 ± 0.24 | 0.83 ± 0.24 | |
| 100 Hz firing probability | AP1 (n = 7) | AP2 (n = 7) | AP3 (n = 7) | AP4 (n = 7) | — | |||
| 1 ± 0 | 1 ± 0 | 0.85 ± 0.37 | 1 ± 0 | |||||
Interneurons: spot diameter 10–15 μm, illumination duration 2–3 ms, power range: 0.05–0.17 mW/μm2. Pyramidal cells: spot diameter 15–20 μm, illumination duration 3–10 ms, power range: 0.09–0.16 mW/μm2.