Figure 3. T6SS1 mediates Vpr bactericidal activity.

• A–C
  Viability counts of Escherichia coli (A, B) and Vibrio parahaemolyticus POR1/Δhcp1 (C) prey before (0 h) and after (4 h) co‐culture with indicated Vpr strains on media containing 3% NaCl at 30°C. Asterisks mark statistical significance between samples at 4‐h timepoint by unpaired, two‐tailed Student's t‐test (*P < 0.05); n.s., no significant difference; WT, wild‐type.
• D
  Expression (Cells) and secretion (Media) of VgrG1 were detected by immunoblot using specific antibodies against VgrG1. Loading control (LC) is shown for total protein lysate.

Data information: In (B–D), attackers contain either an empty expression vector (Empty) or vector for arabinose‐inducible expression of TssG1 (pTssG1). Source data are available online for this figure.